Elucidating the in vivo interactome of HIV-1 RNA by hybridization capture and mass spectrometry.

ABSTRACT: HIV-1 replication requires myriad interactions between cellular proteins and the viral unspliced RNA. These interactions are important in archetypal RNA processes such as transcription and translation as well as for more specialized functions including alternative splicing and packaging of unspliced genomic RNA into virions. We present here a hybridization capture strategy for purification of unspliced full-length HIV RNA-protein complexes preserved in vivo by formaldehyde crosslinking, and coupled with mass spectrometry to identify HIV RNA-protein interactors in HIV-1 infected cells. One hundred eighty-nine proteins were identified to interact with unspliced HIV RNA including Rev and Gag/Gag-Pol, 24 host proteins previously shown to bind segments of HIV RNA, and over 90 proteins previousl

SUBMITTER: Knoener RA 

PROVIDER: S-EPMC5717263 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature