Cellular v-ATPase is required for virion assembly compartment formation in human cytomegalovirus infection.

ABSTRACT: Successful generation of virions from infected cells is a complex process requiring orchestrated regulation of host and viral genes. Cells infected with human cytomegalovirus (HCMV) undergo a dramatic reorganization of membrane organelles resulting in the formation of the virion assembly compartment, a process that is not fully understood. Here we show that acidification of vacuoles by the cellular v-ATPase is a crucial step in the formation of the virion assembly compartment and disruption of acidification results in mis-localization of virion components and a profound reduction in infectious virus levels. In addition, knockdown of ATP6V0C blocks the increase in nuclear size, normally associated with HCMV infection. Inhibition of the v-ATPase does not affect intracellular levels of viral

SUBMITTER: Pavelin J 

PROVIDER: S-EPMC5717334 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature