Pho dynamically interacts with Spt5 to facilitate transcriptional switches at the hsp70 locus.

ABSTRACT:

Background

Numerous target genes of the Polycomb group (PcG) are transiently activated by a stimulus and subsequently repressed. However, mechanisms by which PcG proteins regulate such target genes remain elusive.

Results

We employed the heat shock-responsive hsp70 locus in Drosophila to study the chromatin dynamics of PRC1 and its interplay with known regulators of the locus before, during and after heat shock. We detected mutually exclusive binding patterns for HSF and PRC1 at the hsp70 locus. We found that Pleiohomeotic (Pho), a DNA-binding PcG member, dynamically interacts with Spt5, an elongation factor. The dynamic interaction switch between Pho and Spt5 is triggered by the recruitment of HSF to chromatin. Mutation in the protein-protein interaction domain (REPO domain

SUBMITTER: Pereira A 

PROVIDER: S-EPMC5718073 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature