Selecting the right gate to identify relevant cells for your assay: a study of thioglycollate-elicited peritoneal exudate cells in mice.

ABSTRACT:

Objective

In this study, we investigate the diversity and modulation of leukocyte populations represented in the gates defined by size and granularity at different time points of thioglycollate-induced peritonitis in mouse.

Results

The inflammatory cells were distributed into four regions (R1-R4) of a data plot graph defined by cell size and granularity. R1 and R2 contained agranular cells that were small in size and predominately included T (CD3⁺) lymphocytes along with B (B220⁺) lymphocytes. Macrophages (F4/80⁺) were the predominant cells found in the R3 region. However, these cells were present in all regions, albeit at a lower frequency in R1 and R2. Granulocytes (Gr1⁺) were mainly distributed in R3 and R4. The wide distributio

SUBMITTER: Hermida MDR 

PROVIDER: S-EPMC5718147 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature