Structure and dynamics of the multi-domain resuscitation promoting factor RpfB from Mycobacterium tuberculosis.

ABSTRACT: RpfB is multidomain protein that is crucial for Mycobacterium tuberculosis resuscitation from dormancy. This protein cleaves cell wall peptidoglycan, an essential bacterial cell wall polymer formed by glycan chains of β-(1-4)-linked-N-acetylglucosamine (GlcNAc) and N-acetylmuramic acid (MurNAc) cross-linked by short peptide stems. RpfB is structurally complex being composed of five distinct domains, namely a catalytic, a G5 and three DUF348 domains. Here, we have undertaken a combined experimental and computation structural investigations on the entire protein to gain insights into its structure-function relationships. CD spectroscopy and light scattering experiments have provided insights into the protein fold stability and into its oligomeric state. Using the available structure informat

SUBMITTER: Ruggiero A 

PROVIDER: S-EPMC5718287 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature