Permeable Protein-Loaded Polymersome Cascade Nanoreactors by Polymerization-Induced Self-Assembly.

ABSTRACT: Enzyme loading of polymersomes requires permeability to enable them to interact with the external environment, typically requiring addition of complex functionality to enable porosity. Herein, we describe a synthetic route towards intrinsically permeable polymersomes loaded with functional proteins using initiator-free visible light-mediated polymerization-induced self-assembly (photo-PISA) under mild, aqueous conditions using a commercial monomer. Compartmentalization and retention of protein functionality was demonstrated using green fluorescent protein as a macro-molecular chromophore. Catalytic enzyme-loaded vesicles using horseradish peroxidase and glucose oxidase were also prepared and the permeability of the membrane towards their small molecule substrates was revealed for the first

SUBMITTER: Blackman LD 

PROVIDER: S-EPMC5718297 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature