Single Amino Acid Variant Profiles of Subpopulations in the MCF-7 Breast Cancer Cell Line.

ABSTRACT: Cancers are initiated and developed from a small population of stem-like cells termed cancer stem cells (CSCs). There is heterogeneity among this CSC population that leads to multiple subpopulations with their own distinct biological features and protein expression. The protein expression and function may be impacted by amino acid variants that can occur largely due to single nucleotide changes. We have thus performed proteomic analysis of breast CSC subpopulations by mass spectrometry to study the presence of single amino acid variants (SAAVs) and their relation to breast cancer. We have used CSC markers to isolate pure breast CSC subpopulation fractions (ALDH+ and CD44+/CD24- cell populations) and the mature luminal cells (CD49f-EpCAM+) from the MCF-7 breast cancer cell line. By searchin

SUBMITTER: Tan Z 

PROVIDER: S-EPMC5718353 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature