Oligonucleotide modifications enhance probe stability for single cell transcriptome in vivo analysis (TIVA).

ABSTRACT: Single cell transcriptomics provides a powerful discovery tool for identifying new cell types and functions as well as a means to probe molecular features of the etiology and treatment of human diseases, including cancer. However, such analyses are limited by the difficulty of isolating mRNA from single cells within biological samples. We recently introduced a photochemical method for isolating mRNA from single living cells, Transcriptome In Vivo Analysis (TIVA). The TIVA probe is a "caged" polyU : polyA oligonucleotide hairpin designed to enter live tissue, where site-specific activation with 405 nm laser reveals the polyU-biotin strand to bind mRNA in a target cell, enabling subsequent mRNA isolation and sequencing. The TIVA method is well suited for analysis of living cells in resected

SUBMITTER: Yeldell SB 

PROVIDER: S-EPMC5718921 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature