Engineering an aldehyde dehydrogenase toward its substrates, 3-hydroxypropanal and NAD⁺, for enhancing the production of 3-hydroxypropionic acid.

ABSTRACT: 3-Hydroxypropionic acid (3-HP) can be produced via the biological route involving two enzymatic reactions: dehydration of glycerol to 3-hydroxypropanal (3-HPA) and then oxidation to 3-HP. However, commercial production of 3-HP using recombinant microorganisms has been hampered with several problems, some of which are associated with the toxicity of 3-HPA and the efficiency of NAD⁺ regeneration. We engineered α-ketoglutaric semialdehyde dehydrogenase (KGSADH) from Azospirillum brasilense for the second reaction to address these issues. The residues in the binding sites for the substrates, 3-HPA and NAD⁺, were randomized, and the resulting libraries were screened for higher activity. Isolated KGSADH variants had significantly lower K_m values for both the subs

SUBMITTER: Park YS 

PROVIDER: S-EPMC5719400 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature