Simple protocol for population (Sanger) sequencing for Zika virus genomic regions.

ABSTRACT:

Background

A number of Zika virus (ZIKV) sequences were obtained using Next-generation sequencing (NGS), a methodology widely applied in genetic diversity studies and virome discovery. However Sanger method is still a robust, affordable, rapid and specific tool to obtain valuable sequences.

Objective

The aim of this study was to develop a simple and robust Sanger sequencing protocol targeting ZIKV relevant genetic regions, as envelope protein and nonstructural protein 5 (NS5). In addition, phylogenetic analysis of the ZIKV strains obtained using the present protocol and their comparison with previously published NGS sequences were also carried out.

Methods

Six Vero cells isolates from serum and one urine sample were available to develop the procedure. Primer sets were

SUBMITTER: Cabral GB 

PROVIDER: S-EPMC5719533 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature