Study of stem cell homing & self-renewal marker gene profile of ex vivo expanded human CD34⁺ cells manipulated with a mixture of cytokines & stromal cell-derived factor 1.

ABSTRACT:

Background & objectives

Next generation transplantation medicine aims to develop stimulating cocktail for increased ex vivo expansion of primitive hematopoietic stem and progenitor cells (HSPC). The present study was done to evaluate the cocktail GF (Thrombopoietin + Stem Cell factor + Flt3-ligand) and homing-defining molecule Stromal cell-derived factor 1 (SDF1) for HSPC ex vivo expansion.

Methods

Peripheral blood stem cell (n=74) harvests were analysed for CD34hiCD45lo HSPC. Immunomagnetically enriched HSPC were cultured for eight days and assessed for increase in HSPC, colony forming potential in vitro and in vivo engrafting potential by analyzing human CD45+ cells. Expression profile of genes for homing and stemness were studied using microarray analysis. Expression of a

SUBMITTER: Kode J 

PROVIDER: S-EPMC5719609 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature