Fusion of Regionally Specified hPSC-Derived Organoids Models Human Brain Development and Interneuron Migration.

ABSTRACT: Organoid techniques provide unique platforms to model brain development and neurological disorders. Whereas several methods for recapitulating corticogenesis have been described, a system modeling human medial ganglionic eminence (MGE) development, a critical ventral brain domain producing cortical interneurons and related lineages, has been lacking until recently. Here, we describe the generation of MGE and cortex-specific organoids from human pluripotent stem cells that recapitulate the development of MGE and cortex domains, respectively. Population and single-cell RNA sequencing (RNA-seq) profiling combined with bulk assay for transposase-accessible chromatin with high-throughput sequencing (ATAC-seq) analyses revealed transcriptional and chromatin accessibility dynamics and lineage rel

SUBMITTER: Xiang Y 

PROVIDER: S-EPMC5720381 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature