Molecular Rationale for Improved Dynamic Nuclear Polarization of Biomembranes.

ABSTRACT: Dynamic nuclear polarization (DNP) enhanced solid-state NMR can provide orders of magnitude in signal enhancement. One of the most important aspects of obtaining efficient DNP enhancements is the optimization of the paramagnetic polarization agents used. To date, the most utilized polarization agents are nitroxide biradicals. However, the efficiency of these polarization agents is diminished when used with samples other than small molecule model compounds. We recently demonstrated the effectiveness of nitroxide labeled lipids as polarization agents for lipids and a membrane embedded peptide. Here, we systematically characterize, via electron paramagnetic (EPR), the dynamics of and the dipolar couplings between nitroxide labeled lipids under conditions relevant to DNP applications. Compleme

SUBMITTER: Smith AN 

PROVIDER: S-EPMC5720678 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature