Low Efficiency Upconversion Nanoparticles for High-Resolution Coalignment of Near-Infrared and Visible Light Paths on a Light Microscope.

ABSTRACT: The combination of near-infrared (NIR) and visible wavelengths in light microscopy for biological studies is increasingly common. For example, many fields of biology are developing the use of NIR for optogenetics, in which an NIR laser induces a change in gene expression and/or protein function. One major technical barrier in working with both NIR and visible light on an optical microscope is obtaining their precise coalignment at the imaging plane position. Photon upconverting particles (UCPs) can bridge this gap as they are excited by NIR light but emit in the visible range via an anti-Stokes luminescence mechanism. Here, two different UCPs have been identified, high-efficiency micro⁵⁴⁰-UCPs and lower efficiency nano⁵⁴⁵-UCPs, that respond to NIR light and emit visib

SUBMITTER: Sundaramoorthy S 

PROVIDER: S-EPMC5720688 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature