Spatial transcriptomes within the Pseudomonas aeruginosa biofilm architecture.

ABSTRACT: Bacterial cooperative associations and dynamics in biofilm microenvironments are of special interest in recent years. Knowledge of localized gene-expression and corresponding bacterial behaviors within the biofilm architecture at a global scale has been limited, due to a lack of robust technology to study limited number of cells in stratified layers of biofilms. With our recent pioneering developments in single bacterial cell transcriptomic analysis technology, we generated herein an unprecedented spatial transcriptome map of the mature in vitro Pseudomonas aeruginosa biofilm model, revealing contemporaneous yet altered bacterial behaviors at different layers within the biofilm architecture (i.e., surface, middle and interior of the biofilm). Many genes encoding unknown functions were high

SUBMITTER: Heacock-Kang Y 

PROVIDER: S-EPMC5720903 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature