Functional validation and expression analysis of myotubes converted from skin fibroblasts using a simple direct reprogramming strategy.

ABSTRACT: Previously, we reported that MyoD, a master gene for myogenic cells, could efficiently convert primary skin fibroblasts into myoblasts and myotubes, thereby effecting direct reprogramming. In this study, we further demonstrated that MyoD-expressing primary fibroblasts displayed rapid movement in culture, with a movement velocity that was significantly faster, almost four times, than mouse primary myoblasts. MyoD-transduced cells obtained the characteristics of Ca2 + release and electrically-stimulated contraction, which was comparable to C2C12 myotubes, suggesting that the essential features of muscle were observed in the transduced cells. Furthermore, the ability to fuse to the host myoblasts means that gene transfer from MyoD-transduced cells to host muscle cells could be obtained by cel

SUBMITTER: Horio F 

PROVIDER: S-EPMC5721582 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature