Excitable Dynamics and Yap-Dependent Mechanical Cues Drive the Segmentation Clock.

ABSTRACT: The periodic segmentation of the vertebrate body axis into somites, and later vertebrae, relies on a genetic oscillator (the segmentation clock) driving the rhythmic activity of signaling pathways in the presomitic mesoderm (PSM). To understand whether oscillations are an intrinsic property of individual cells or represent a population-level phenomenon, we established culture conditions for stable oscillations at the cellular level. This system was used to demonstrate that oscillations are a collective property of PSM cells that can be actively triggered in vitro by a dynamical quorum sensing signal involving Yap and Notch signaling. Manipulation of Yap-dependent mechanical cues is sufficient to predictably switch isolated PSM cells from a quiescent to an oscillatory state in vitro, a beha

SUBMITTER: Hubaud A 

PROVIDER: S-EPMC5722254 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature