Zebrafish skeleton development: High resolution micro-CT and FIB-SEM block surface serial imaging for phenotype identification.

ABSTRACT: Although bone is one of the most studied living materials, many questions about the manner in which bones form remain unresolved, including fine details of the skeletal structure during development. In this study, we monitored skeleton development of zebrafish larvae, using calcein fluorescence, high-resolution micro-CT 3D images and FIB-SEM in the block surface serial imaging mode. We compared calcein staining of the skeletons of the wild type and nacre mutants, which are transparent zebrafish, with micro-CT for the first 30 days post fertilization embryos, and identified significant differences. We quantified the bone volumes and mineral contents of bones, including otoliths, during development, and showed that such developmental differences, including otolith development, could be helpf

SUBMITTER: Silvent J 

PROVIDER: S-EPMC5722281 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature