A systematic approach to evaluate the influence of environmental conditions on eDNA detection success in aquatic ecosystems.

ABSTRACT: The use of environmental DNA (eDNA) to determine the presence and distribution of aquatic organisms has become an important tool to monitor and investigate freshwater communities. The successful application of this method in the field, however, is dependent on the effectiveness of positive DNA verification, which is influenced by site-specific environmental parameters. Factors affecting eDNA concentrations in aquatic ecosystems include flow conditions, and the presence of substances that possess DNA-binding properties or inhibitory effects. In this study we investigated the influence of different environmental parameters on the detection success of eDNA using the invasive goby Neogobius melanostomus. In a standardized laboratory setup, different conditions of flow, sediment-properties, and

SUBMITTER: Stoeckle BC 

PROVIDER: S-EPMC5722286 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature