Absence of anti-hypocretin receptor 2 autoantibodies in post pandemrix narcolepsy cases.

ABSTRACT:

Background

A recent publication suggested molecular mimicry of a nucleoprotein (NP) sequence from A/Puerto Rico/8/1934 (PR8) strain, the backbone used in the construction of the reassortant strain X-179A that was used in Pandemrix® vaccine, and reported on anti-hypocretin (HCRT) receptor 2 (anti-HCRTR2) autoantibodies in narcolepsy, mostly in post Pandemrix® narcolepsy cases (17 of 20 sera). In this study, we re-examined this hypothesis through mass spectrometry (MS) characterization of Pandemrix®, and two other pandemic H1N1 (pH1N1)-2009 vaccines, Arepanrix® and Focetria®, and analyzed anti-HCRTR2 autoantibodies in narcolepsy patients and controls using three independent strategies.

Methods

MS characterization of Pandemrix® (2 batches), Arepanrix® (4 batches) and Focetria®

SUBMITTER: Luo G 

PROVIDER: S-EPMC5722318 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature