Analysis of the interactome of Schistosoma mansoni histone deacetylase 8.

ABSTRACT:

Background

Histone deacetylase 8 from Schistosoma mansoni (SmHDAC8) is essential to parasite growth and development within the mammalian host and is under investigation as a target for the development of selective inhibitors as novel schistosomicidal drugs. Although some protein substrates and protein partners of human HDAC8 have been characterized, notably indicating a role in the function of the cohesin complex, nothing is known of the partners and biological function of SmHDAC8.

Methodology/principal findings

We therefore employed two strategies to characterize the SmHDAC8 interactome. We first used SmHDAC8 as a bait protein in yeast two-hybrid (Y2H) screening of an S. mansoni cDNA library. This allowed the identification of 49 different sequences encoding proteins. We ne

SUBMITTER: Caby S 

PROVIDER: S-EPMC5722368 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature