Base-Resolution Mapping Reveals Distinct m¹A Methylome in Nuclear- and Mitochondrial-Encoded Transcripts.

ABSTRACT: Gene expression can be post-transcriptionally regulated via dynamic and reversible RNA modifications. N¹-methyladenosine (m¹A) is a recently identified mRNA modification; however, little is known about its precise location and biogenesis. Here, we develop a base-resolution m¹A profiling method, based on m¹A-induced misincorporation during reverse transcription, and report distinct classes of m¹A methylome in the human transcriptome. m¹A in 5' UTR, particularly those at the mRNA cap, associate with increased translation efficiency. A different, small subset of m¹A exhibit a GUUCRA tRNA-like motif, are evenly distributed in the transcriptome, and are dependent on the methyltransferase TRMT6/61A. Additionally, we sho

SUBMITTER: Li X 

PROVIDER: S-EPMC5722686 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature