A screening system to identify transcription factors that induce binding site-directed DNA demethylation.

ABSTRACT:

Background

DNA methylation is a fundamental epigenetic modification that is involved in many biological systems such as differentiation and disease. We and others recently showed that some transcription factors (TFs) are involved in the site-specific determination of DNA demethylation in a binding site-directed manner, although the reports of such TFs are limited.

Results

Here, we develop a screening system to identify TFs that induce binding site-directed DNA methylation changes. The system involves the ectopic expression of target TFs in model cells followed by DNA methylome analysis and overrepresentation analysis of the corresponding TF binding motif at differentially methylated regions. It successfully identified binding site-directed demethylation of SPI1, which is kno

SUBMITTER: Suzuki T 

PROVIDER: S-EPMC5723091 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature