Virtual Screening and Prediction of Binding of Caprine CSN1S2 Protein Tryptic Peptides to Glucokinase.

ABSTRACT:

Introduction

Glucokinase (hexokinase D) is an enzyme that phosphorylates glucose in hepatocytes totrap it in the cell and prime it for conversion to other compounds, yet this enzyme has low affinity to bind with glucose. In Diabetes Mellituspatients, the blood glucose level is poorly controled.

Material and methods

This study explored the possibility to induce glucokinase activity with bioactive peptides derived from the goat milk protein CSN1S2 by in- silico docking approach. Two bioactive tryptic peptides, CSN1S2 residues 41-47 and 214-221, were successfully docked to glucokinase and found to bind to the activation site.

Results

Amino acid residues Asn41, Ala43, His45 and Arg221 from these peptides provided the major contribution to docking to glucokinase. Asn41 mad

SUBMITTER: Fatchiyah F 

PROVIDER: S-EPMC5723197 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature