Thermal-Stability and Reconstitution Ability of Listeria Phages P100 and A511.

ABSTRACT: The study evaluated the thermal-stability of Listeria phages P100 and A511 at temperatures simulating the preparation of ready-to-eat meats. The phage infectivity after heating to 71°C and holding for a minimum of 30 s, before eventually cooling to 4°C were examined. Higher temperatures of 75, 80, and 85°C were also tested to evaluate their effect on phages thermal-stability. This study found that despite minor differences in the amino acid sequences of their structural proteins, the two phages responded differently to high temperatures. P100 activity declined at least 10 log (PFU mL^-1) with exposure to 71°C (30 s) and falling below the limit of detection (1 log PFU mL^-1) while, A511 dropped from 10⁸ to 10⁵ PFU mL^-1. Cooling res

SUBMITTER: Ahmadi H 

PROVIDER: S-EPMC5723416 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature