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Probing the excited-state chemical shifts and exchange parameters by nitrogen-decoupled amide proton chemical exchange saturation transfer (HN^dec-CEST).

ABSTRACT: CEST-NMR spectroscopy is a powerful tool for probing the conformational dynamics of macromolecules. We present a HN^dec-CEST experiment that simplifies the relaxation matrix, reduces fitting parameters, and enhances signal resolution. Importantly, fitting of HN^dec-CEST profiles enables robust extraction of exchange rates as well as excited-state chemical shifts and populations.

SUBMITTER: Wu Q

PROVIDER: S-EPMC5723528 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature

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Probing the excited-state chemical shifts and exchange parameters by nitrogen-decoupled amide proton chemical exchange saturation transfer (HN^dec-CEST).

OmicsDI is part of the ELIXIR infrastructure

Tweets

Dataset Information

Probing the excited-state chemical shifts and exchange parameters by nitrogen-decoupled amide proton chemical exchange saturation transfer (HNdec-CEST).

OmicsDI is part of the ELIXIR infrastructure

Tweets

Probing the excited-state chemical shifts and exchange parameters by nitrogen-decoupled amide proton chemical exchange saturation transfer (HN^dec-CEST).