An expression screen for aged-dependent microRNAs identifies miR-30a as a key regulator of aging features in human epidermis.

ABSTRACT: The mechanisms affecting epidermal homeostasis during aging remain poorly understood. To identify age-related microRNAs, a class of non-coding RNAs known to play a key role in the regulation of epidermal homeostasis, an exhaustive miRNA expression screen was performed in human keratinocytes from young or elderly subjects. Many microRNAs modulated by aging were identified, including miR-30a, in which both strands were overexpressed in aged cells and epidermal tissue. Stable MiR-30a over-expression strongly impaired epidermal differentiation, inducing severe barrier function defects in an organotypic culture model. A significant increase was also observed in the level of apoptotic cells in epidermis over-expressing miR-30a. Several gene targets of miR-30a were identified in keratinocytes, in

SUBMITTER: Muther C 

PROVIDER: S-EPMC5723692 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature