The E3 ubiquitin ligase SCF^FBXL14 complex stimulates neuronal differentiation by targeting the Notch signaling factor HES1 for proteolysis.

ABSTRACT: Molecular oscillators are important cellular regulators of, for example, circadian clocks, oscillations of immune regulators, and short-period (ultradian) rhythms during embryonic development. The Notch signaling factor HES1 (hairy and enhancer of split 1) is a well-known repressor of proneural genes, and HES1 ultradian oscillation is essential for keeping cells in an efficiently proliferating progenitor state. HES1 oscillation is driven by both transcriptional self-repression and ubiquitin-dependent proteolysis. However, the E3 ubiquitin ligase targeting HES1 for proteolysis remains unclear. Based on siRNA-mediated gene silencing screening, co-immunoprecipitation, and ubiquitination assays, we discovered that the E3 ubiquitin ligase SCF^FBXL14 complex regulates HES1 ubiquitinati

SUBMITTER: Chen F 

PROVIDER: S-EPMC5723999 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature