Single-subunit oligosaccharyltransferases of <i>Trypanosoma brucei</i> display different and predictable peptide acceptor specificities.

ABSTRACT: Trypanosoma brucei causes African trypanosomiasis and contains three full-length oligosaccharyltransferase (OST) genes; two of which, TbSTT3A and TbSTT3B, are expressed in the bloodstream form of the parasite. These OSTs have different peptide acceptor and lipid-linked oligosaccharide donor specificities, and trypanosomes do not follow many of the canonical rules developed for other eukaryotic N-glycosylation pathways, raising questions as to the basic architecture and detailed function of trypanosome OSTs. Here, we show by blue-native gel electrophoresis and stable isotope labeling in cell culture proteomics that the TbSTT3A and TbSTT3B proteins associate with each other in large complexes that contain no other detectable protein subunits. We prob

SUBMITTER: Jinnelov A 

PROVIDER: S-EPMC5724017 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature