Prediction of Protein Complexes in <i>Trypanosoma brucei</i> by Protein Correlation Profiling Mass Spectrometry and Machine Learning.

ABSTRACT: A disproportionate number of predicted proteins from the genome sequence of the protozoan parasite Trypanosoma brucei, an important human and animal pathogen, are hypothetical proteins of unknown function. This paper describes a protein correlation profiling mass spectrometry approach, using two size exclusion and one ion exchange chromatography systems, to derive sets of predicted protein complexes in this organism by hierarchical clustering and machine learning methods. These hypothesis-generating proteomic data are provided in an open access online data visualization environment (http://134.36.66.166:8083/complex_explorer). The data can be searched conveniently via a user friendly, custom graphical interface. We provide examples of both potential new subunits of known protein com

SUBMITTER: Crozier TWM 

PROVIDER: S-EPMC5724185 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature