Transcriptome-wide characterization of human cytomegalovirus in natural infection and experimental latency.

ABSTRACT: The transcriptional program associated with herpesvirus latency and the viral genes regulating entry into and exit from latency are poorly understood and controversial. Here, we developed and validated a targeted enrichment platform and conducted large-scale transcriptome analyses of human cytomegalovirus (HCMV) infection. We used both an experimental hematopoietic cell model of latency and cells from naturally infected, healthy human subjects (clinical) to define the breadth of viral genes expressed. The viral transcriptome derived from experimental infection was highly correlated with that from clinical infection, validating our experimental latency model. These transcriptomes revealed a broader profile of gene expression during infection in hematopoietic cells than previously appreciate

SUBMITTER: Cheng S 

PROVIDER: S-EPMC5724264 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature