Germline Cas9 expression yields highly efficient genome engineering in a major worldwide disease vector, Aedes aegypti.

ABSTRACT: The development of CRISPR/Cas9 technologies has dramatically increased the accessibility and efficiency of genome editing in many organisms. In general, in vivo germline expression of Cas9 results in substantially higher activity than embryonic injection. However, no transgenic lines expressing Cas9 have been developed for the major mosquito disease vector Aedes aegypti Here, we describe the generation of multiple stable, transgenic Ae. aegypti strains expressing Cas9 in the germline, resulting in dramatic improvements in both the consistency and efficiency of genome modifications using CRISPR. Using these strains, we disrupted numerous genes important for normal morphological development, and even generated triple mutants from a single injection. We have also managed to incr

SUBMITTER: Li M 

PROVIDER: S-EPMC5724270 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature