On the effective depth of viral sequence data.

ABSTRACT: Genome sequence data are of great value in describing evolutionary processes in viral populations. However, in such studies, the extent to which data accurately describes the viral population is a matter of importance. Multiple factors may influence the accuracy of a dataset, including the quantity and nature of the sample collected, and the subsequent steps in viral processing. To investigate this phenomenon, we sequenced replica datasets spanning a range of viruses, and in which the point at which samples were split was different in each case, from a dataset in which independent samples were collected from a single patient to another in which all processing steps up to sequencing were applied to a single sample before splitting the sample and sequencing each replicate. We conclude that n

SUBMITTER: Illingworth CJR 

PROVIDER: S-EPMC5724399 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature