Silk fibroin scaffolds with muscle-like elasticity support in vitro differentiation of human skeletal muscle cells.

ABSTRACT: Human adult skeletal muscle has a limited ability to regenerate after injury and therapeutic options for volumetric muscle loss are few. Technologies to enhance regeneration of tissues generally rely upon bioscaffolds to mimic aspects of the tissue extracellular matrix (ECM). In the present study, silk fibroins from four Lepidoptera (silkworm) species engineered into three-dimensional scaffolds were examined for their ability to support the differentiation of primary human skeletal muscle myoblasts. Human skeletal muscle myoblasts (HSMMs) adhered, spread and deposited extensive ECM on all the scaffolds, but immunofluorescence and quantitative polymerase chain reaction analysis of gene expression revealed that myotube formation occurred differently on the various scaffolds. Bombyx mori fibr

SUBMITTER: Chaturvedi V 

PROVIDER: S-EPMC5724504 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature