Lipopolysaccharide structure impacts the entry kinetics of bacterial outer membrane vesicles into host cells.

ABSTRACT: Outer membrane vesicles are nano-sized microvesicles shed from the outer membrane of Gram-negative bacteria and play important roles in immune priming and disease pathogenesis. However, our current mechanistic understanding of vesicle-host cell interactions is limited by a lack of methods to study the rapid kinetics of vesicle entry and cargo delivery to host cells. Here, we describe a highly sensitive method to study the kinetics of vesicle entry into host cells in real-time using a genetically encoded, vesicle-targeted probe. We found that the route of vesicular uptake, and thus entry kinetics and efficiency, are shaped by bacterial cell wall composition. The presence of lipopolysaccharide O antigen enables vesicles to bypass clathrin-mediated endocytosis, which enhances both their entry

SUBMITTER: O'Donoghue EJ 

PROVIDER: S-EPMC5724897 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature