N⁶-methyladenosine (m⁶A) recruits and repels proteins to regulate mRNA homeostasis.

ABSTRACT: RNA modifications are integral to the regulation of RNA metabolism. One abundant mRNA modification is N⁶-methyladenosine (m⁶A), which affects various aspects of RNA metabolism, including splicing, translation and degradation. Current knowledge about the proteins recruited to m⁶A to carry out these molecular processes is still limited. Here we describe comprehensive and systematic mass-spectrometry-based screening of m⁶A interactors in various cell types and sequence contexts. Among the main findings, we identified G3BP1 as a protein that is repelled by m⁶A and positively regulates mRNA stability in an m⁶A-regulated manner. Furthermore, we identified FMR1 as a sequence-context-dependent m⁶A reader, thus revealing a

SUBMITTER: Edupuganti RR 

PROVIDER: S-EPMC5725193 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature