Computational flow cytometry analysis reveals a unique immune signature of the human maternal-fetal interface.

ABSTRACT:

Problem

Decidual immune dysregulation is thought to underlie major pregnancy disorders; however, incomplete understanding of the decidual immune interface has hampered the mechanistic investigation.

Method of study

Human term decidua was collected, and single-cell phenotypic information was acquired by highly polychromatic flow cytometry. Cellular identity analysis was performed with t-distributed stochastic neighbor embedding, DensVM clustering, and matched to CellOntology database.

Results

Traditional analytical methods validated known cellular T and dendritic cell subsets in human term decidua. Computational analysis revealed a complex and tissue-specific decidual immune signature in both the innate and adaptive immune compartments.

Conclusion

Polychromatic

SUBMITTER: Vazquez J 

PROVIDER: S-EPMC5725254 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature