Bioreactor model of neuromuscular junction with electrical stimulation for pharmacological potency testing.

ABSTRACT: In vitro models of the neuromuscular junction (NMJ) are emerging as a valuable tool to study synaptogenesis, synaptic maintenance, and pathogenesis of neurodegenerative diseases. Many models have previously been developed using a variety of cell sources for skeletal muscle and motoneurons. These models can advanced by integrating beneficial features of the native developmental milieu of the NMJ. We created a functional in vitro model of NMJ by bioreactor cultivation of transdifferentiated myocytes and stem cell-derived motoneurons, in the presence of electrical stimulation. In conjunction with a coculture medium, electrical stimulation resulted in improved maturation and function of motoneurons and myocytes, as evidenced by mature cellular structures, increased expression of neuronal and m

SUBMITTER: Charoensook SN 

PROVIDER: S-EPMC5725265 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature