Selection, characterization, and thermal stabilization of llama single domain antibodies towards Ebola virus glycoprotein.

ABSTRACT:

Background

A key advantage of recombinant antibody technology is the ability to optimize and tailor reagents. Single domain antibodies (sdAbs), the recombinantly produced variable domains derived from camelid and shark heavy chain antibodies, provide advantages of stability and solubility and can be further engineered to enhance their properties. In this study, we generated sdAbs specific for Ebola virus envelope glycoprotein (GP) and increased their stability to expand their utility for use in austere locals. Ebola virus is extremely virulent and causes fatal hemorrhagic fever in ~ 50 percent of the cases. The viral GP binds to host cell receptors to facilitate viral entry and thus plays a critical role in pathogenicity.

Results

An immune phage display library containing mo

SUBMITTER: Liu JL 

PROVIDER: S-EPMC5726015 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature