Different phenotypic and molecular mechanisms associated with multidrug resistance in Gram-negative clinical isolates from Egypt.

ABSTRACT:

Objectives

We set out to investigate the prevalence, different mechanisms, and clonal relatedness of multidrug resistance (MDR) among third-generation cephalosporin-resistant Gram-negative clinical isolates from Egypt.

Materials and methods

A total of 118 third-generation cephalosporin-resistant Gram-negative clinical isolates were included in this study. Their antimicrobial susceptibility pattern was determined using Kirby-Bauer disk diffusion method. Efflux pump-mediated resistance was tested by the efflux-pump inhibitor-based microplate assay using chlorpromazine. Detection of different aminoglycoside-, β-lactam-, and quinolone-resistance genes was done using polymerase chain reaction. The genetic diversity of MDR isolates was investigated using random amplification of po

SUBMITTER: Helmy OM 

PROVIDER: S-EPMC5726372 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature