Filling reference gaps via assembling DNA barcodes using high-throughput sequencing-moving toward barcoding the world.

ABSTRACT: Over the past decade, biodiversity researchers have dedicated tremendous efforts to constructing DNA reference barcodes for rapid species registration and identification. Although analytical cost for standard DNA barcoding has been significantly reduced since early 2000, further dramatic reduction in barcoding costs is unlikely because Sanger sequencing is approaching its limits in throughput and chemistry cost. Constraints in barcoding cost not only led to unbalanced barcoding efforts around the globe, but also prevented high-throughput sequencing (HTS)-based taxonomic identification from applying binomial species names, which provide crucial linkages to biological knowledge. We developed an Illumina-based pipeline, HIFI-Barcode, to produce full-length Cytochrome c oxidase subunit I (COI)

SUBMITTER: Liu S 

PROVIDER: S-EPMC5726475 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature