In-depth PtdIns(3,4,5)P₃ signalosome analysis identifies DAPP1 as a negative regulator of GPVI-driven platelet function.

ABSTRACT: The class I phosphoinositide 3-kinase (PI3K) isoforms play important roles in platelet priming, activation, and stable thrombus formation. Class I PI3Ks predominantly regulate cell function through their catalytic product, the signaling phospholipid phosphatidylinositol 3,4,5-trisphosphate [PtdIns(3,4,5)P₃], which coordinates the localization and/or activity of a diverse range of binding proteins. Notably, the complete repertoire of these class I PI3K effectors in platelets remains unknown, limiting mechanistic understanding of class I PI3K-mediated control of platelet function. We measured robust agonist-driven PtdIns (3,4,5)P₃ generation in human platelets by lipidomic mass spectrometry (MS), and then used affinity-capture coupled to high-resolution proteomic MS to

SUBMITTER: Durrant TN 

PROVIDER: S-EPMC5726495 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature