Improved sample treatment protocol for accurate detection of live Salmonella spp. in food samples by viability PCR.

ABSTRACT: Culture-based detection is still considered as the standard way for detection of Salmonella in foods, although molecular methods, such as viability PCR (vPCR), have been introduced to overcome some disadvantages of traditional culture methods. Despite the success of the vPCR methodology, the problem of false-positive results is a major drawback, especially when applied to environmental samples, hindering the interpretation of the results. To improve the efficiency of vPCR, many approaches have been introduced by several authors during the last years. In the present work, the combination of PEMAX dye, double tube change, and double photo-activation step was established as a strategy to improve vPCR protocol. By combining these approaches, we developed an improved sample treatment protocol a

SUBMITTER: Dinh Thanh M 

PROVIDER: S-EPMC5726647 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature