Quiescence Exit of Tert⁺ Stem Cells by Wnt/β-Catenin Is Indispensable for Intestinal Regeneration.

ABSTRACT: Fine control of stem cell maintenance and activation is crucial for tissue homeostasis and regeneration. However, the mechanism of quiescence exit of Tert⁺ intestinal stem cells (ISCs) remains unknown. Employing a Tert knockin (Tert^TCE/+) mouse model, we found that Tert⁺ cells are long-term label-retaining self-renewing cells, which are partially distinguished from the previously identified +4 ISCs. Tert⁺ cells become mitotic upon irradiation (IR) injury. Conditional ablation of Tert⁺ cells impairs IR-induced intestinal regeneration but not intestinal homeostasis. Upon IR injury, Wnt signaling is specifically activated in Tert⁺ cells via the ROS-HIFs-transactivated Wnt2b signaling axis. Importantly, conditional knockout of β

SUBMITTER: Suh HN 

PROVIDER: S-EPMC5726811 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature