Pathologic Stimulus Determines Lineage Commitment of Cardiac C-kit⁺ Cells.

ABSTRACT:

Background

Although cardiac c-kit⁺ cells are being tested in clinical trials, the circumstances that determine lineage differentiation of c-kit⁺ cells in vivo are unknown. Recent findings suggest that endogenous cardiac c-kit⁺ cells rarely contribute cardiomyocytes to the adult heart. We assessed whether various pathological stimuli differentially affect the eventual cell fates of c-kit⁺ cells.

Methods

We used single-cell sequencing and genetic lineage tracing of c-kit⁺ cells to determine whether various pathological stimuli would result in different fates of c-kit⁺ cells.

Results

Single-cell sequencing of cardiac CD45^-c-kit⁺ cells showed innate heterogeneity, indicative of the exi

SUBMITTER: Chen Z 

PROVIDER: S-EPMC5726921 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature