Enhancing size based size separation through vertical focus microfluidics using secondary flow in a ridged microchannel.

ABSTRACT: High throughput size based separation and sorting of bioparticles and cells is critical to a variety of biomedical processing steps for medical diagnostics and pharmaceutical purification. Improving microfluidic size-based particle/cell sorting is a challenge to better address the need for generating more homogeneous subpopulations for study and use. We propose a novel advance to microfluidic sorting devices that uses three-dimensional focusing of the sample to optimally position particles to amplify the size-dependent differences in trajectories caused by differential secondary flows. The result is an increase in the purity of small particles by 35- fold and large particles by 8-fold in comparison to unfocused flow. Our simulated and experimental data reveal for the first time that positi

SUBMITTER: Tasadduq B 

PROVIDER: S-EPMC5727182 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature