Dopamine D₂ receptor signalling controls inflammation in acute pancreatitis via a PP2A-dependent Akt/NF-κB signalling pathway.

ABSTRACT:

Background and purpose

Dopamine has multiple anti-inflammatory effects, but its role and molecular mechanism in acute pancreatitis (AP) are unclear. We investigated the role of dopamine signalling in the inflammatory response in AP.

Experimental approach

Changes in pancreatic dopaminergic system and effects of dopamine, antagonists and agonists of D₁ and D₂ dopamine receptors were analysed in wild-type and pancreas-specific Drd2^-/- mice with AP (induced by caerulein and LPS or L-arginine) and pancreatic acinar cells with or without cholecystokinin (CCK) stimulation. The severity of pancreatitis was assessed by measuring serum amylase and lipase and histological assessments. The NF-κB signalling pathway was evaluated, and macrophage and neutro

SUBMITTER: Han X 

PROVIDER: S-EPMC5727253 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature