Immuno-pharmacological characterization of group II metabotropic glutamate receptors controlling glutamate exocytosis in mouse cortex and spinal cord.

ABSTRACT:

Background and purpose

We recently proposed the existence of mGlu₃ -preferring autoreceptors in spinal cord terminals and of mGlu₂ -preferring autoreceptors in cortical terminals. This study aims to verify our previous conclusions and to extend their pharmacological characterization.

Experimental approach

We studied the effect of LY566332, an mGlu₂ receptor positive allosteric modulator (PAM), and of LY2389575, a selective mGlu₃ receptor negative allosteric (NAM) modulator, on the mGlu_2/3 agonist LY379268-mediated inhibition of glutamate exocytosis [measured as KCl-evoked release of preloaded [³ H]-D-aspartate]. The mGlu₂ PAM BINA and the mGlu₃ NAM ML337, as well as selective antibodies r

SUBMITTER: Olivero G 

PROVIDER: S-EPMC5727332 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature