Structural insight into catalytic mechanism of PET hydrolase.

ABSTRACT: PET hydrolase (PETase), which hydrolyzes polyethylene terephthalate (PET) into soluble building blocks, provides an attractive avenue for the bioconversion of plastics. Here we present the structures of a novel PETase from the PET-consuming microbe Ideonella sakaiensis in complex with substrate and product analogs. Through structural analyses, mutagenesis, and activity measurements, a substrate-binding mode is proposed, and several features critical for catalysis are elucidated.

SUBMITTER: Han X 

PROVIDER: S-EPMC5727383 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature