Data-Independent Mass Spectrometry Approach for Screening and Identification of DNA Adducts.

ABSTRACT: Long-term exposures to environmental toxicants and endogenous electrophiles are causative factors for human diseases including cancer. DNA adducts reflect the internal exposure to genotoxicants and can serve as biomarkers for risk assessment. Liquid chromatography-multistage mass spectrometry (LC-MSⁿ) is the most common method for biomonitoring DNA adducts, generally targeting single exposures and measuring up to several adducts. However, the data often provide limited evidence for a role of a chemical in the etiology of cancer. An "untargeted" method is required that captures global exposures to chemicals, by simultaneously detecting their DNA adducts in the genome; some of which may induce cancer-causing mutations. We established a wide selected ion monitoring tandem mass spec

SUBMITTER: Guo J 

PROVIDER: S-EPMC5727898 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature